| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 41 | Latilactobacillus sakei | AGCAACCGTCTTGGCACA | CGCGACCATCCATGACAAC | 59.81 | 59.28 | 269 | 115 |
100.00%
|
100.00%
|
| Primer 42 | Latilactobacillus sakei | AGCAACCGTCTTGGCACA | ATCGCGACCATCCATGACA | 59.81 | 59.18 | 271 | 115 |
100.00%
|
100.00%
|
| Primer 43 | Latilactobacillus sakei | TGAGCGCAGTTGGTTGGT | TGTTGGTGGGCAAATGGG | 59.81 | 58.12 | 262 | 115 |
100.00%
|
100.00%
|
| Primer 44 | Latilactobacillus sakei | AGCTGCTGCGGTTTTCAG | CGCGTCAGCAATCGTTTGT | 58.65 | 59.80 | 235 | 115 |
100.00%
|
100.00%
|
| Primer 45 | Latilactobacillus sakei | TGCAGCAGCCATTATCGC | TGGCTTGGCGTTGCTTTTC | 58.58 | 59.93 | 167 | 114 |
100.00%
|
100.00%
|
| Primer 46 | Latilactobacillus sakei | TGGCGACCTCACGATTCAC | ACTGTGCGCACATCACCA | 60.08 | 59.89 | 183 | 113 |
100.00%
|
100.00%
|
| Primer 47 | Latilactobacillus sakei | AAGACGCCAAGGGTCAACC | ACTGTGCGCACATCACCA | 60.23 | 59.89 | 249 | 113 |
100.00%
|
100.00%
|
| Primer 48 | Latilactobacillus sakei | TTGGGGATGCTGGTATGCC | TTCGATTGGTGTCTCCCCA | 59.77 | 58.23 | 206 | 113 |
100.00%
|
100.00%
|
| Primer 49 | Latilactobacillus sakei | ACGCAGCGAAGAAGCCAT | AGCGCTGCAATATCCCGT | 60.05 | 59.49 | 202 | 112 |
100.00%
|
100.00%
|
| Primer 50 | Latilactobacillus sakei | TGACGCAACGACAAGGCA | TGCGAACGCGTTACTCGT | 60.20 | 60.05 | 193 | 112 |
100.00%
|
100.00%
|